Molecular Immunology Lab
In the Molecular Immunology Lab, we conduct experiments related to signal transduction, epigenetics, cancer mechanisms and gene transcription in the mammalian immune system. In this lab, we perform molecular biology experiments using techniques such as molecular cloning, construction of bacterial artificial chromosomes, and yeast two hybrid screening for protein-DNA and protein-protein interactions. Since 2013, we have been using genome editing tools such as TALENs and Crispr/Cas9 to introduce mutations into the genomes of tissue culture cells and induced pluripotent stem cells. In addition to various basic molecular biology equipment, the lab accommodates a spectrophotometer for nucleic acid, protein and cell growth measurements and two PCR machines which have independently controlled dual blocks for running four simultaneous protocols. The lab has tissue culture facilities and is equipped with laminar flow cabinets, CO2 incubators, and an inverted phase-contrast microscope.
We routinely use a the Roche Lightcycler realtime PCR machine, the computer-controlled Fluoroscan Ascent fluorometer for both luminometric and fluorometric measurements, the Neon electroporator for transfecting tissue culture cells, the two laser, 6-color BD FACSCanto flow cytometer, the Zeiss LSM 710 confocal microscope, a Zeiss Axio Observer inverted microscope fitted with a Pecon live cell incubation chamber with CO2 and temperature control and the Xcelligence realtime tissue culture analysis device (the latter three machines are housed in the new nanotechnology center (SUNUM)). The tissue culture research in this lab is conducted in the FENS building as well as the new lentivirus tissue culture facility in SUNUM. We are very interested in conducting live cell microscopy of tissue culture cells expressing various fluorescent proteins localized to different sub-cellular organelles. We also perform chromatin immunoprecipitation using the Diagenode Bioruptor sonicator.